Zhao Lab

Murray, C., Ontiveros, C., Wentworth, J., Blinkiewicz, P.V., Leung, B., Bai, H., Spicer, N., Holtz, A., Tanner, C., Balasubramanian, A., Li, W., Dray, E., Zhao, W., Tyler Curiel, T.

BMJ Journal for Immunotherapy of Cancer

Telmisartan increases olaparib efficacy in homologous recombination proficient tumors by augmenting type I interferon production.

Rawal, Y., Kwon, Y., Jia, L., Ruben, E., Tovar, M., Fang, Q., Jamalruddin, M. A., Zhou, S., Kuppa, S., Syed, S., Jasper, A., Katz, J., Nayak, D., Stratton, C., Rogers, C., Kaur, H., Ji, J.-H., Samentar, L., Zhao, W., Dray, E., Taylor, A., Burma, S., Rao, M., Libich, D., Hromas, R., Mazin, A., Zhang, F., Jasin, M., Zhou, D., Bernstein, K., Greene, E., Wasmuth, E., Sung, P., Olsen. K. S..

Nat Struct Mol Biol

Cryo-EM Structures of RAD51-X3C and RAD51-X3CDX2 Reveal Activation of Presynaptic Filament Assembly by Paralog Exchange.

Dutta, A.*, Ji, J.*, Fang, Q., Zhou, S. Liang, F., Nyalwidhe, J., McDonald, D., Verway-Cohen, A., De La Pena Avalos, B., Herrero, A.O., Li, W., Fitzgerald, O., Taylor, A.B., Dray, E., Kwon, Y., Semmes, J.O., Tsai, M., Hromas, R., Burma, S., Bishop, A.J., Mazin, A., Wasmuth, E., Olsen, S., Zhou, D. Zhao, W.#, Kupfer, G.#, Sung, P.#.

Nat Struct Mol Biol

Mechanism of SETX-BRCA1-BARD1 complex in resolution of R-loops and transcription-replication conflicts.

Li, W.*, Wu, B.*, Gao, B., Irvin, E.M., Ghosh, A., Eliaz, L., Huang, Y., Kwon, Y., Stiefel, C. M., Nguyen, T., Zhao, D., Suarez, H. J., Ni, T., Alejo, S., Fitzgerald, O., Song, X., Wasmuth, E.V., Zheng, S., Leung, J., Xue, X., Wang, H., Ji, J. #, Lan, L. #, and Zhao, W#.

Nature Communications

HELZ-BRCA2 Complex Resolves R-loops to Dive Transcriptional-coupled Homologous Recombination.

Katz. J. N., Samentar, L., Fang, Q., Li, W., Ji, J., Gao, Q., Kuppa, S., Gao, B.,, Rogers, C., Kaur, H., Badamchi, A., Syed, S., Tomimatsu, N., Mukherjee, B., Irvin, E., Zhou, S., Libich, D., Hromas, R., Mazin, A., Rao, M., Olsen, S., Wasmuth, E., Losada, A., Zhou, D., Wang, H., Lan, L., Greene, E., Zhao, W., Kwon, Y., Burma, S., Sung, P.

Nature Communications

Multi-faceted roles of PDS5B in RAD51-dependent homology-directed DNA repair and replication fork protection.

Tomimatsu, N., Cristofaro, L., Kanji, S., Samentar, L., Jordan, B.R., Kittler, R., Habib, A.A., Espindola-Netto, J.M., Tchkonia, T., Kirkland,J.L., Burns, T.C., Sarkaria, J.N., Gilbert, A., Floyd, J.R., Hromas. R., Zhao, W., Zhou, D., Sung, P., Mukherjee, B., Burma, S.

EMBO Molecular Medicine

Targeting cIAP2 in a novel senolytic strategy prevents glioblastoma recurrence after radiotherapy.

Neal, F.E., Li, W., Uhrig, M.E., Katz, J.N., Syed, S., Sharma, N., Dutta, A., Burma, S., Hromas, R., Mazin, A.V., Dray, E., Libich, D.S., Olsen, S.K., Wasmuth, E.V., Zhao, W., Sørensen, C.S., Wiese, C., Kwon, Y., Sung, P..

Cell Reports

Distinct roles of the two BRCA2 DNA-binding domains in DNA damage repair and replication fork preservation.

Rogers, C.M., Kaur, H., Swift, M.L., Raina, V.B., Zhou, S., Ajinkya S Kawale, A.S., Syed, S., Kelly, K.G., Jasper, A.M., Salunkhe, S., Kwon, Y., Wang, J., Shabestari, A.B., Daley, J.M., Sacks, A., Gaczynska, M.E., Osmulski, P.A., Rawal, Y., Tomimatsu, N., Gayther, S.A., Lawrenson, K., Burma, S., Wasmuth, E.V., Olsen, S.K., Zhao, W., Hromas, R., Libich, D.S., Mazin, A.V., Zhou, D., E Greene,E.C., Chowdhury, D., Sung, P..

Science

CTC1-STN1-TEN1 controls DNA break repair pathway choice via DNA end resection blockade.

Yang, H., Gao, B., Wu, B., Li, W., Zhu, X., Phoon, L., Luo, J., Gui, F., Zhao, W., Jia, L., and Lan, L.

Cell Death and Differentiation

cGAS restricts PARP1-mediated microhomology-mediated end joining by suppressing poly-ADP-ribosylation.

Kelliher, J., Folkerts, M., Shen, K., Song, W., Tengler, K., Stiefel, C., Lee, S., Dray, E., Zhao, W., Koss, B., Pannunzio, N., Leung, J..

Nature Communications

Evolved histone tail regulates 53BP1damaged chromatin recruitment.

Salunkhe, S. *, Daley, J.M. *, Xue, C. *, Tomimatsu, N. *, Kaur, H. *, Raina, V., Jasper, A. M., Rogers, C.M., Li, W., Zhou, S., Mojidra, R., Kwon, Y., Dinh, H., Mukherjee, B., Habib, A.A., Hromas, R., Mazin, A.V., Wasmuth, E.V., Olsen, S.K., Libich, D.S., Zhou, D., Zhao, W., Greene, E.C. #, Burma, S. #, and Sung, P. #

Nature

Promotion of DNA End Resection by the BRCA1-BARD1 Tumor Suppressor in Homologous Recombination.

Huang, Y.*, Li, W.*, Ji, J., Tomimatsu, N., Foo, T., Lan, L., Burma, S, Xia, B., and Zhao, W.

Nature Communications

DSS1 Restrains BRCA2's Engagement with dsDNA for Homologous Recombination, Replication Fork Protection, and R-loop Homeostasis.

Fitzgerald, O., Wu B., Wang M., Maqbool, R., Li, W. #, Zhao, W. #.

STAR Protocols

Protocol for evaluating the E3 Ligase Activity of BRCA1-BARD1 and its Variants by Nucleosomal Histone Ubiquitylation.

Wang, M.*, Li, W.*, Tomimatsu, N., Yu, C., Ji, J., Alejo, S., Witus, S., Alimbetov, D., Fitzgerald, O., Bo, W., Wang, Q., Huang, Y., Gan, Y., Dong, F., Kwon, Y., Sareddy, G., Curiel, T., Habib, A., Hromas, R., Ivanov, D., Brzovic, P., Burma, S.#, Klevit, R.#, and Zhao, W.#

Mol. Cell

Crucial Roles of the BRCA1-BARD1 E3 Ubiquitin Ligase Activity in Homology-Directed DNA Repair.

Witus, S., Tuttle, L., Li, W, Zelter, A., W, W., Kermoade, K., Wilburn, D., Davis, T., Brzovic P.#, Zhao, W.#, Klevit, R.#

EMBO Journal

BRCA1/BARD1 intrinsically disordered regions facilitate chromatin recruitment and ubiquitylation.

Alejo, S., Palacios, E. B., Venkata, P.P., He, Y., Li, W., Johnson D.J., Chen, Y., Jayamohan, S., Uday P. Pratap, P.U., Clarke, K., Zou, Y., Lv, Y., Weldon, K., Viswanadhapalli, S., Lai, Z., Ye, Z., Chen, Y., Gilbert, R.A., Suzuki, T., Tekmal, R.R., Zhao, W., Zheng, S., Vadlamudi, K.R., Brenner, J.A., R. Sareddy, R.G.

Lysine-specific histone demethylase 1A (KDM1A/LSD1) inhibition attenuates DNA double-strand breaks repair and augments the efficacy of temozolomide in glioblastoma

Kwon, Y.*, Rösner, H.*, Zhao, W., Selemenakis, P., He, Z., Kawale, A., Katz, J., Rogers, C., Neal, F., Badamchi, A., Petrosius, V., Singh, A., Joel, M., Lu ,L., Holloway, S., Burma, S., Mukherjee, B., Hromas, R., Mazin, A., Wiese, C.#, Sørensen, C.#, Sung, P.

DNA binding and RAD51 interaction by the BRCA2 C-terminus orchestrate DNA repair and replication fork preservation.

Chung, S., Kang, M., Alimbetov, D, Mun, G., Yunn, N., Kim, Y., Kim, B., Wie, M., Lee, E., Ra, J., Oh, J., Lee, D., Lee, K., Kim, J., Han, S., Kim, K., Chung, W., Nam, K., Park, N., Lee, B., Kim, S., Zhao, W., Ryu, S., Lee, Y., Myung, K., Cho, Y.

Regulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase

Witus S., Zhao W., Brzovic P., Klevit R.

BRCA1/BARD1 is a nucleosome reader and writer

Kornepati A.V.R., Boyd J.T., Murray C.E., Saifetiarova J., Avalos B.P., Rogers C.M., Haiyan Bai, Padron A.S., Liao Y., Ontiveros C., Svatek R.S., Hromas R., Li R., Hu Y., Conejo-Garcia J.R., Vadlamudi R.K., Zhao W., Dray E., Sung P., Curiel T.J.

Tumor-intrinsic programmed death-ligand 1 promotes DNA repair by homologous recombination in distinct cancers and can suppress PARP inhibitor synthetic lethality

Wang M., Rogers C.M., Alimbetov D.S., Zhao W., ...

In Vitro Reconstitution of BRCA1-BARD1/RAD51 Mediated Homologous DNA Pairing

Foo T.K., Vincelli G., Huselid E., Her J., Zheng H., Simhadri S., Wang M., Huo Y., Li T., Yu X., Li H., Zhao W., Bunting S.F., Xia B.

ATR and ATM phosphorylation of BRCA1 at T1394 promotes homologous recombinational repair and G2/M checkpoint maintenance

E Pires, N Sharma, P Selemenakis, B Wu, Y Huang, DS Alimbetov, ...

RAD51AP1 mediates RAD51 activity through nucleosome interaction

SR Witus, AL Burrell, DP Farrell, J Kang, M Wang, JM Hansen, A Pravat, ...

BRCA1/BARD1 site-specific ubiquitylation of nucleosomal H2A is directed by BARD1

C Xue,L Molnarova,JB Steinfeld,W Zhao,C Ma,M Spirek,K Kaniecki,...

Single-molecule visualization of human RECQ5 interactions with single-stranded DNA recombination intermediates

DG Maranon,N Sharma,Y Huang,P Selemenakis,M Wang,N Altina,...

NUCKS1 promotes RAD54 activity in homologous recombination DNA repair

F Liang,AS Miller,C Tang,D Maranon,EA Williamson,R Hromas,...

The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair

JB Steinfeld,O Beláň,Y Kwon,T Terakawa,A Al-Zain,MJ Smith,...

Defining the influence of Rad51 and Dmc1 lineage-specific amino acids on genetic recombination

G Srinivasan,EA Williamson,K Kong,AS Jaiswal,G Huang,HS Kim,...

MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers

W Zhao,C Wiese,Y Kwon,R Hromas,P Sung

The BRCA tumor suppressor network in chromosome damage repair by homologous recombination

T Rao,S Longerich,W Zhao,H Aihara,P Sung,Y Xiong

Importance of homo-dimerization of Fanconi-associated nuclease 1 in DNA flap cleavage

S Welty,Y Teng,Z Liang,W Zhao,LH Sanders,JT Greenamyre,...

RAD52 is required for RNA-templated recombination repair in post-mitotic neurons

L Zhao,J Xu,W Zhao,P Sung,HW Wang

Determining the RAD51-DNA nucleoprotein filament structure and function by cryo-electron microscopy

W Zhao,JB Steinfeld,F Liang,X Chen,DG Maranon,CJ Ma,Y Kwon,...

Promotion of RAD51-mediated homologous DNA pairing by BRCA1-BARD1

W Zhao,JB Steinfeld,F Liang,X Chen,DG Maranon,C Jian Ma,Y Kwon,...

BRCA1-BARD1 promotes RAD51-mediated homologous DNA pairing

J Xu,L Zhao,Y Xu,W Zhao,P Sung,HW Wang

Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange

MV Borgogno,MR Monti,W Zhao,P Sung,CE Argaraña,RJ Pezza

Tolerance of DNA mismatches in Dmc1 recombinase-mediated DNA strand exchange

KS Trego,T Groesser,AR Davalos,AC Parplys,W Zhao,MR Nelson,...

Non-catalytic Roles for XPG with BRCA1 and BRCA2 in Homologous Recombination and Genome Stability

AC Parplys,W Zhao,N Sharma,T Groesser,F Liang,DG Maranon,...

NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability

JY Lee,T Terakawa,Z Qi,JB Steinfeld,S Redding,YH Kwon,WA Gaines,...

Base triplet stepping by the Rad51/RecA family of recombinases

W Zhao,S Vaithiyalingam,J San Filippo,DG Maranon,J Jimenez-Sainz,...

Promotion of BRCA2-dependent homologous recombination by DSS1 via RPA targeting and DNA mimicry

W Zhao,P Sung

Significance of ligand interactions involving Hop2-Mnd1 and the RAD51 and DMC1 recombinases in homologous DNA repair and XX ovarian dysgenesis

H Moktan,MF Guiraldelli,CA Eyster,W Zhao,CY Lee,T Mather,...

Solution structure and DNA-binding properties of the winged helix domain of the meiotic recombination HOP2 protein

R Buisson,J Niraj,J Pauty,R Maity,W Zhao,Y Coulombe,P Sung,...

Breast cancer proteins PALB2 and BRCA2 stimulate polymerase η in recombination-associated DNA synthesis at blocked replication forks

W Zhao,D Saro,M Hammel,YH Kwon,Y Xu,RP Rambo,GJ Williams,...

Mechanistic insights into the role of Hop2–Mnd1 in meiotic homologous DNA pairing

MH Dunlop,E Dray,W Zhao,J San Filippo,MS Tsai,SG Leung,D Schild,...

Mechanistic insights into RAD51-associated protein 1 (RAD51AP1) action in homologous DNA repair

MH Dunlop,E Dray,W Zhao,MS Tsai,C Wiese,D Schild,P Sung

RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif

Y Kwon,W Zhao,P Sung

Biochemical studies on human Rad51-mediated homologous recombination

H Niu,WH Chung,Z Zhu,Y Kwon,W Zhao,P Chi,R Prakash,C Seong,...

Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae

RB Robertson,DN Moses,YH Kwon,P Chan,W Zhao,P Chi,H Klein,...

Visualizing the disassembly of S. cerevisiae Rad51 nucleoprotein filaments

S Raynard,W Zhao,W Bussen,L Lu,YY Ding,V Busygina,AR Meetei,...

Functional role of BLAP75 in BLM-topoisomerase IIIα-dependent Holliday junction processing

W Zhao,Z Ye,J Zhao

RbrA, a cyanobacterial rubrerythrin, functions as a FNR-dependent peroxidase in heterocysts in protection of nitrogenase from damage by hydrogen peroxide in Anabaena sp. PCC 7120

Y Zhang,H Pu,Q Wang,S Cheng,W Zhao,Y Zhang,J Zhao

PII is important in regulation of nitrogen metabolism but not required for heterocyst formation in the cyanobacterium Anabaena sp. PCC 7120

W Zhao,Q Guo,J Zhao

A membrane-associated Mn-superoxide dismutase protects the photosynthetic apparatus and nitrogenase from oxidative damage in the cyanobacterium Anabaena sp. PCC 7120

X Chen,D Cui,C Liu,H Li,W Zhao

Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

B Hu,G Yang,W Zhao,Y Zhang,J Zhao

MreB is important for cell shape but not for chromosome segregation of the filamentous cyanobacterium Anabaena sp. PCC 7120

Y Shi,W Zhao,W Zhang,Z Ye,J Zhao

Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120

Y Zhao,Y Shi,W Zhao,X Huang,D Wang,N Brown,J Brand,J Zhao

CcbP, a calcium-binding protein from Anabaena sp. PCC 7120, provides evidence that calcium ions regulate heterocyst differentiation